Location: Home > Basic Medical >
-
Studies on Protection and Immune Mechanism of Recombinant Bb-ESAT-6-MPT64 Vaccine of Mycobacterium Tu
ObjectiveTo compare the protective immunity and immune mechanism in BALB/c mice by immunization with the recombinant rBb-ESAT-6-MPT64 vaccines against subsequent challenge with H37Ra by different inoculation routines, and to measure the leve....
-
Preparation and Characterization of Monoclonal Antibody Against M Protein and NS1 Protein of Dengue V
Objectives:The global prevalence of dengue virus has grown dramatically in recent decades, predominantly in Southeast Asia areas.Dengue fever (DF) and its more serious forms, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are....
-
Comparison in the Effects of IL-2, IL-12, IL-15 and IFNα on Gene Regulation of Granzymes of Human NK
ObjectiveHuman natural killer cells are cytotoxic lymphocytes that are responsible for inducing rapid apoptosis of virus infected cells or tumors.They play cytolytic activity through several distinct pathways,including Fas-L,TRAIL,and perfor....
-
Functional Characterization of Interleukin-15 Gene Transduction into the Human Natural Killer Cell Li
ObjectiveNatural killer (NK) cells are a key component of the innate immune system that is involved in the early defense against virus-infected or transformed cells by non-major histocompatibility complex (MHC)-, non-T-cell receptor (TCR)-re....
-
Regulation of Baicalin for Toll-like Receptors of the Lymphocytes
Objective: To study the effect of baicalin on Toll-like receptors(TLR)of the lymphocytes and function of immune regulation.Methods: 1. The T and B lymphoytes of human were separated by nylon wool cotton column. 2. UF-50 was used to analyze t....
-
The Biological Characteristics and the Proliferative Effect on Dendritic Cells of Decidual Stroma Cel
【Objective】1.To develop a simple and satisfactory method to culture mice decidual stromal cell(DSC). The construction of a co-coltured system of decidual cells will lay a good foundation to study pathological pregnancy.2. To establish me....
-
The Preparation of Monoclonal Antibody to Fba of GAS and the Primary Identification of Its Epitope of
Objective: Group A streptococcus (GAS) is a common bacterial pathogen of human which can cause the throat, skin, and soft tissue infection. It is also a cause of children toxic diseases such as scarlet fever, toxic shock symptom. Meanwhile t....
-
In Vitro Studies on DC-inducing Effect of FLT3-L and GM-CSF and on the Methods for Culturing DC-CIK
Objectives:Along with the development of the cellular immunology and molecular biology, cell-mediated immunotherapy have been developed in the clinical experiment, and have got significant curative effect. Cytokine Induced Killer cells (CIK)....
-
Research on Prevention and Treat Graft Versus Host Disease by Donor Immature Dendritic Cells
Objective: Based on the generated mechanism for graft-versus-host disease (GVHD), establishing the modulating model of dendritic cell induced lymphocytes in vitro, to observe the ability of immature dendritic cells that induce the stimulatio....
-
Experiment Study about the Bifidobacterium and the Whole Peptidoglycan of Bifidobacterium Effection o
【Objective】To explore in vitro the effect of Bifidobacterium and the Whole Peptidoglycan of Bifidobacterium (WPG) on proliferation, maturation and immunoregulation of DCs from the cord blood monocyte, investigate the mechanism of Bifidob....
-
Preparation and Characterization of Monoclonal Antibody Against HBV S Protein
Objectives: Hepatitis B virus is a very dangerous pathogen for human health. Till now, there is no effective therapeutic methods and solutions for chronic Hepatitis B virus infection. HBsAg is a sign of infecting HBV, and the first signal ap....
-
Recombine a Bifidobacterium Based Oral Live Vaccine Expression Escherichia Coli Colonization Factor A
Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease worldwide. It remains the first major causes of diarrheal morbidity and infant mortality in developing countries (second to Rotavirus), and is al....
-
Antitumor Effect of DC Vaccine Modified by HSP70-H22 Tumor Peptide Complex
Objective: To study the method of constitution DC vaccine modified by Hsp70-H22 tumor peptide complex,to observe the cytokine IL-10,IL-12 secreted by DC, the surface marker CD11c,CD40,CD80 expressed on DC and the antitumor effect in vitro .M....
-
Study on the Preparation and Anti-Tumor Biological Effects of Dendritic Cell-Renal Cell Carcinoma 786
Objective:To prepare and select pure Dendritic cell-Renal cell carcinoma 786-O cell line fusion vaccine(DC-RCC786-O),By researching its biological characters and anti-tumor effects in vitro, we hoped to provide the theory foundation and expe....
-
Preparation and Identification of Monoclonal Antibodies Against PreS2 of Hepatitis B Virus
Objective:Previous studies have show that the protein of HBV pre S2 have the receptor of Polymenized Human Serum Albumin (PHSA), which could mediat the DANE partical's absorption into the surface of the hepatocytes and made important role in....
-
Pilot Study of Gene Vaccine of Helicobacter Pylori Urea Channel Protein (UreI)
Helicobacter pylori (H. pylori) is a major pathogen of chronic active gastritis and peptic ulcer, and plays an important role in the pathogenesis of gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. H. pylori is unique i....
-
The Study of Effect of Interleukin-10 on Renal Tubular Epithelial to Mesenchymal Transition and Relev
Objective To investigate the effect of interleukin-10(IL-10) on epithelial to mesenchymal transition in human renal tubular cells and relevant mechanisms.Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. These....
-
Immunological Function Analysis of Bifidobacterium Based Vaccine Expression ETEC-LTB
Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease worldwide. It remains major causes of diarrheal morbidity and infant mortality in developing countries, and is also pathogenic bacteria for trave....
-
Impact of Chronic Restraint Stress on Splenocyte Immunity, Growth and Angiogenesis of Mouse Forestoma
BACKGROUND&OBJECTIVE: Recent study found psychosocial factors play some important roles in carcinogenesis and development of malignant tumors, but its mechanisms remain unclear. This study was to investigate the impact of chronic restraint s....
-
Studies on Construction and Immune Mechanism of Recombinant Bb-EmⅡ/3-Em14-3-3 Vaccine of Echinococc
ObjectiveTo construct the recombinant Bb-EmⅡ/3-Em14-3-3 vaccine with Echinococcus multilocularisⅡ/3 and 14-3-3 antigen coding gene; To analyse the expression of Escherichia.coli-Bifidobacterium shuttle plasmid pGEX-EmⅡ/3-Em14-3-3 induc....